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KMID : 0950020030290020045
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Journal of Health Science & Medical Technology
2003 Volume.29 No. 2 p.45 ~ p.50
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Proteomic analysis of plasma proteines from patients with Obstructive sleep apnea
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Lee Seung-Gwan
Lee Chang-Kyu
Yoon Dae-We
Sung Euen-Me
Kim Jin-Kwan
Shin Chol
Lee Sung-Ho
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Abstract
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Obstructive Sleep Apnea(OSA) is characterized by episode of complete or partial pharyngeal obstruction during sleep and deeply related to cardiovascular disease and cerebrovascular disease. Many studies show that Hypoxia, Hypertension, and the production of superoxide produced by elevated homocysteine concentration may damage to vascular endothelium. In this study we performed proteomic-based approach to characterize the plasma protein associated with wound healing mechanism. First, overnight polysomnographic study was carried out in sleep laboratory, using standard recording techniques. Five cases of OSA patients and 5 normal controls were analyzed by two dimensional gel electrophoresis (pH 3-10NL, 8-18% gradient). Protein spots were cut from 2 -D gels stained by Coomasie blue, and were identified by using matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS). The results were compared with those of paired normal controls. Comparative analysis of the respective spot patterns in 2DE shows that Vitronectin which plays an important role in repairing wounded vessel was increased. Following protein was also increased in OSA patients : Ig alpha-1 chain C region
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